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Chromatography on Sephadex LH20 as an efficient purification step after removal of internucleotide 2,2,2-trichloroethyl protective groups from oligoribonucleotide phosphotriesters.

Chromatography on Sephadex LH20, in a linear gradient of methanol in 0.02M TEAB buffer pH 7.5, is proposed as a fast and efficient method for the isolation and purification of protected oligoribonucleotide phosphodiesters obtained by deprotection of internucleotide phosphotriesters, and for the moni...

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Main Authors: Grześkowiak, K, Adamiak, R W, Wiewiórowski, M
Formato: Artigo
Idioma:Inglês
Publicado: 1980
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC323975/
https://ncbi.nlm.nih.gov/pubmed/7443541
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