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In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus

We have cloned full-length double-stranded cDNAs of tobacco mosaic virus (TMV) (tomato strain L) RNA into a transcription vector, pPM1, which facilitates the correct transcription initiation from the first nucleotide of the inserted double-stranded cDNA, corresponding to the 5′ end of TMV RNA. When...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Meshi, Tetsuo, Ishikawa, Masayuki, Motoyoshi, Fusao, Semba, Kentaro, Okada, Yoshimi
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Biological Sciences: Biochemistry
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323886/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593727/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.14.5043
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