Establishment of Methylation-Specific PCR for the Mouse p53 Gene

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker...

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Main Authors: Okazaki, Ryuji, Ootsuyama, Akira, Yoshida, Yasuhiro, Norimura, Toshiyuki
Formato: Artigo
Idioma:Inglês
Publicado: SAGE-Hindawi Access to Research 2011
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3238407/
https://ncbi.nlm.nih.gov/pubmed/22203902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/938435
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