Isolation of ras GTP-binding mutants using an in situ colony-binding assay.

We have developed a strategy to isolate mutant ras genes encoding proteins defective in GTP binding. Random in vitro mutagenesis of a v-Harvey (Ha)-ras expression vector was followed by an in situ GTP-binding assay on lysed bacterial colonies. Single amino acid substitutions at ras codon 83, 119, or...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Feig, L A, Pan, B T, Roberts, T M, Cooper, G M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1986
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3088563/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.13.4607
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