ロード中...

Isolation of ras GTP-binding mutants using an in situ colony-binding assay.

We have developed a strategy to isolate mutant ras genes encoding proteins defective in GTP binding. Random in vitro mutagenesis of a v-Harvey (Ha)-ras expression vector was followed by an in situ GTP-binding assay on lysed bacterial colonies. Single amino acid substitutions at ras codon 83, 119, or...

詳細記述

保存先:
書誌詳細
主要な著者: Feig, L A, Pan, B T, Roberts, T M, Cooper, G M
フォーマット: Artigo
言語:Inglês
出版事項: 1986
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC323790/
https://ncbi.nlm.nih.gov/pubmed/3088563
タグ: タグ追加
タグなし, このレコードへの初めてのタグを付けませんか!