Isolation of ras GTP-binding mutants using an in situ colony-binding assay.

We have developed a strategy to isolate mutant ras genes encoding proteins defective in GTP binding. Random in vitro mutagenesis of a v-Harvey (Ha)-ras expression vector was followed by an in situ GTP-binding assay on lysed bacterial colonies. Single amino acid substitutions at ras codon 83, 119, or...

Full description

Saved in:
Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Feig, L A, Pan, B T, Roberts, T M, Cooper, G M
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323790/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3088563/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.13.4607
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items