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Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

The ribosomal RNA genes (rDNA) of Tetrahymena thermophila macronucleus exist as free linear 21-kilobase molecules that contain replication origins and telomeres. A mutation in this gene confers resistance to the antibiotic paromomycin. We have isolated rDNA from such a mutant (strain p2f), microinje...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Tondravi, M M, Yao, M C
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1986
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323734/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3459180/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.12.4369
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