Regulated gene expression in transfected primary chicken erythrocytes.

We describe a method for studying transient gene expression in primary avian erythroid cells that involves controlled osmotic shock, followed by DNA transfection using DEAE-dextran. Cells treated in this way reproducibly express high levels of chloramphenicol acetyltransferase (CAT) when transfected...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Hesse, J E, Nickol, J M, Lieber, M R, Felsenfeld, G
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1986
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323722/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3459175/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.12.4312
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