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Efficient recovery and sequencing of mutant genes from mammalian chromosomal DNA.

A retroviral shuttle vector was constructed by introducing the Escherichia coli xanthine (guanine) phosphoribosyltransferase gene (gpt) into the pZip-NeoSV(X)1 vector [Cepko, C. L., Roberts, B. E. & Mulligan, R. C. (1984) Cell 37, 1053-1062]. This vector was packaged into infectious virus which then...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Ashman, C R, Jagadeeswaran, P, Davidson, R L
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323512/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3458187/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.10.3356
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