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Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B.

The cysteine proteinase cathepsin B is one member of the lysosomal acid hydrolases. Based on the peptide sequence of rat liver cathepsin B, an oligonucleotide mixture containing 128 different 17-mers was synthesized and used as a probe to screen adult and fetal human liver cDNA libraries. A recombin...

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Bibliographic Details
Main Authors: Fong, D, Calhoun, D H, Hsieh, W T, Lee, B, Wells, R D
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC323416/
https://ncbi.nlm.nih.gov/pubmed/3010323
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