Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.
Anacystis nidulans 6301 has been transformed in the light to ampicillin resistance with the plasmid pBR322. Permeaplasts prepared by 2-hr treatment of cells with lysozyme and EDTA are transformed with a 50-fold higher efficiency than that observed for cells. beta-Lactamase is present in A. nidulans...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323335/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3085098/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.8.2546 |
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