High-Resolution Melting-Curve Analysis of Ligation-Mediated Real-Time PCR for Rapid Evaluation of an Epidemiological Outbreak of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli

Methods for the confirmation of nosocomial outbreaks of bacterial pathogens are complex, expensive, and time-consuming. Recently, a method based on ligation-mediated PCR (LM/PCR) using a low denaturation temperature which produces specific melting-profile patterns of DNA products has been described....

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Detalhes bibliográficos
Main Authors: Woksepp, Hanna, Jernberg, Cecilia, Tärnberg, Maria, Ryberg, Anna, Brolund, Alma, Nordvall, Michaela, Olsson-Liljequist, Barbro, Wisell, Karin Tegmark, Monstein, Hans-Jürg, Nilsson, Lennart E., Schön, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2011
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232983/
https://ncbi.nlm.nih.gov/pubmed/21956981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01042-11
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