Target-Specific Capture Enhances Sensitivity of Electrochemical Detection of Bacterial Pathogens

We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial...

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Autori principali: Patel, Mayank, Gonzalez, Rodrigo, Halford, Colin, Lewinski, Michael A., Landaw, Elliot M., Churchill, Bernard M., Haake, David A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2011
Soggetti:
Bacteriology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232961/
https://ncbi.nlm.nih.gov/pubmed/21940468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01261-11
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