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Cloning and screening with nanogram amounts of immunopurified mRNAs: cDNA cloning and chromosomal mapping of cystathionine beta-synthase and the beta subunit of propionyl-CoA carboxylase.

We have developed conditions for efficient cDNA cloning of nanogram amounts of purified mRNAs coding for cystathionine beta-synthase [L-serine hydro-lyase (adding homocysteine), EC 4.2.1.22] and for the cytosolic precursors of mitochondrial ornithine transcarbamylase (carbamoylphosphate:L-ornithine...

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Publicado en:Proc Natl Acad Sci U S A
Principais autores: Kraus, J P, Williamson, C L, Firgaira, F A, Yang-Feng, T L, Münke, M, Francke, U, Rosenberg, L E
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1986
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323227/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3457373/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.7.2047
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