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Nanodroplet real-time PCR system with laser assisted heating

We report the successful application of low-power (~30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature measurement, and Taqman real-...

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Detalles Bibliográficos
Main Authors: Kim, Hanyoup, Dixit, Sanhita, Green, Christopher J., Faris, Gregory W.
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232056/
https://ncbi.nlm.nih.gov/pubmed/19129891
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