DNA supercoiling of recombinant plasmids in mammalian cells.
We have used chloroquine/agarose gel electrophoresis and a blot-hybridization technique to study the modulation of superhelicity of extrachromosomal DNA in mammalian cells. The high sensitivity of the procedure has allowed us to measure the change in the specific linking difference or superhelical d...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323139/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006061/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.6.1641 |
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