Mutational and in vivo methylation analysis of F-factor PifC protein binding to the pif operator and the region containing the primary origin of mini-F replication.

We have used in vivo methods to identify multiple DNA-binding sites for the negatively autoregulated mini-F replication factor PifC. Sequence analysis of pif operator constitutive mutants, isolated as insensitive to repression by PifC, establishes the structure of pifO. This site contains a 17-base-...

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Detalhes bibliográficos
Main Authors: Miller, J F, Malamy, M H
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC323090/
https://ncbi.nlm.nih.gov/pubmed/3513175
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