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Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that break away from axon terminals when the cortical tissue is homogenized. The SNs retain pre- and postsynaptic characteristics, which makes them usef...

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Detalhes bibliográficos
Main Authors: Westmark, Pamela R., Westmark, Cara J., Jeevananthan, Athavi, Malter, James S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3230173/
https://ncbi.nlm.nih.gov/pubmed/21946781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3196
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