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Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

We developed a method for rapidly generating thermostable enzyme variants. Our strategy is to introduce the gene coding for a given enzyme from a mesophilic organism into a thermophile, Bacillus stearothermophilus. Variants that retain the enzymatic activity at the higher growth temperatures of the...

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Detalhes bibliográficos
Main Authors: Liao, H, McKenzie, T, Hageman, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC322906/
https://ncbi.nlm.nih.gov/pubmed/3003740
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