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Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability

The extreme pH and protease-rich environment of the upper gastrointestinal tract is a major obstacle facing orally-administered protein therapeutics, including antibodies. Through protein engineering, several Clostridium difficile toxin A-specific heavy chain antibody variable domains (V(H)Hs) were...

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Bibliografiske detaljer
Main Authors: Hussack, Greg, Hirama, Tomoko, Ding, Wen, MacKenzie, Roger, Tanha, Jamshid
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3227653/
https://ncbi.nlm.nih.gov/pubmed/22140551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0028218
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