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Preparation and melting of single strand circular DNA loops.

A method for preparation of single strand DNA circles of almost arbitrary sequence is described. By ligating two sticky ended hairpins together a linear duplex is formed, closed at both ends by single stranded loops. The melting characteristics of such loops are investigated using optical absorbance...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Wemmer, D E, Benight, A S
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1985
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322156/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4080549/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.23.8611
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