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Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena: inhibition by methidiumpropyl.EDTA and localization of the major dye binding sites.

The intervening sequence (IVS) excised from the rRNA precursor of Tetrahymena thermophila is converted to a covalently closed circular RNA in the absence of proteins in vitro. This self-catalyzed cyclization reaction is inhibited by the intercalating dye methidiumpropyl.EDTA (MPE; R.P. Hertzberg and...

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Autors principals: Tanner, N K, Cech, T R
Format: Artigo
Idioma:Inglês
Publicat: 1985
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC322085/
https://ncbi.nlm.nih.gov/pubmed/2415924
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