Selective protection of in vitro synthesized cDNA against nucleases by incorporation of phosphorothioate-analogues.
The conditions for the stepwise synthesis of single- (ss) and double-stranded (ds) cDNA using thio-analogues instead of dNTPs are described in this paper. RNA of paramyxovirus Sendai (strain 6/94) serves as template in these experiments. The increased resistance of this alpha S-modified cDNA against...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1985
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322078/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2415922/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.21.7663 |
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