Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid

PURPOSE: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). METHODS: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well a...

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Detalhes bibliográficos
Main Authors: Bruckova, Lenka, Soukup, Tomas, Visek, Benjamin, Moos, Jiri, Moosova, Martina, Pavelkova, Jana, Rezabek, Karel, Kucerova, Lenka, Micuda, Stanislav, Brcakova, Eva, Mokry, Jaroslav
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2011
Assuntos:
Technical Innovations
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3220434/
https://ncbi.nlm.nih.gov/pubmed/21822582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-011-9617-6
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