Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA.

Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis in the absence of salt caused single strand cleavage at specific sites. IMAGES:

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Sawadaishi, K, Miura, K, Ohtsuka, E, Ueda, T, Ishizaki, K, Shinriki, N
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1985
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322037/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2997743/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.20.7183
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!