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Altered DNA conformations detected by mung bean nuclease occur in promoter and terminator regions of supercoiled pBR322 DNA.

Mung bean nuclease was used to probe for recognizable DNA unwinding and unpairing in the plasmid pBR322. In negatively supercoiled DNA, but not relaxed DNA, cleavages occurred preferentially in non-coding regions of the genome. The types of nucleotide sequences cleaved and which non-coding regions w...

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Main Authors: Sheflin, L G, Kowalski, D
格式: Artigo
語言:Inglês
出版: 1985
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC321943/
https://ncbi.nlm.nih.gov/pubmed/2995917
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