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Purification and characterization of DNA polymerase from the archaebacterium Sulfolobus acidocaldarius.

DNA polymerase has been purified about 25,000-fold from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. On SDS-PAGE the enzyme was observed to have a molecular weight of 100 kDa and to be about 90% pure. The native molecular weight was 108 kDa indicating that the enzyme is composed...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Klimczak, L J, Grummt, F, Burger, K J
Format: Artigo
Language:Inglês
Published: Oxford University Press 1985
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC321864/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3927262/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.14.5269
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