Thiol-exchange in DTSSP crosslinked peptides is proportional to cysteine content and precisely controlled in crosslink detection by two-step LC-MALDI MSMS

The lysine-specific crosslinker 3,3′-dithiobis(sulfosuccinimidylpropionate) (DTSSP) is commonly used in the structural characterization of proteins by chemical crosslinking and mass spectrometry and we here describe an efficient two-step LC-MALDI-TOF/TOF procedure to detect crosslinked peptides. Fir...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Lambert, Wietske, Söderberg, Christopher A G, Rutsdottir, Gudrun, Boelens, Wilbert C, Emanuelsson, Cecilia
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Wiley Subscription Services, Inc., A Wiley Company 2011
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3218361/
https://ncbi.nlm.nih.gov/pubmed/21780214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.699
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