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A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods

The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ratios more extreme than 1:1 was investigated. A comparison of nLC-ESI MSMS and nLC-MALDI MSMS analysis routes was performed. A fixed concentration of a standard six protein mix was spiked with two pr...

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Bibliographic Details
Main Authors: Shirran, Sally L., Botting, Catherine H.
Format: Artigo
Language:Inglês
Published: Elsevier 2010
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2880794/
https://ncbi.nlm.nih.gov/pubmed/20230925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2010.03.003
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