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Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Validating interactions between different proteins is vital for investigation of their biological functions on the molecular level. There are several methods, both in vitro and in vivo, to evaluate protein binding, and at least two methods that complement the shortcomings of each other should be con...

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Bibliografiske detaljer
Main Authors: Ueki, Shoko, Lacroix, Benoît, Citovsky, Vitaly
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3217633/
https://ncbi.nlm.nih.gov/pubmed/21860380
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2961
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