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Development of a Single-Tube, Cell Lysis-Based, Genus-Specific PCR Method for Rapid Identification of Mycobacteria: Optimization of Cell Lysis, PCR Primers and Conditions, and Restriction Pattern Analysis

A single-tube PCR method was developed for efficient identification of nontuberculous mycobacteria (NTM) and their environmental isolates in about 3 h without conventional DNA isolation. The following three steps were optimized or developed: (i) a simple, 6-min direct cell lysis protocol as a PCR pr...

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Detalhes bibliográficos
Main Authors: Khan, Izhar U. H., Yadav, Jagjit S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC321669/
https://ncbi.nlm.nih.gov/pubmed/14715804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.1.453-457.2004
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