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Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid

BACKGROUND: Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. However, pol β has not been purified or enzymatically characterized from the model fish species Danio rerio (zebrafish). We used...

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Detalhes bibliográficos
Main Authors: Ishido, Tomomi, Yamazaki, Naoshi, Ishikawa, Mitsuru, Hirano, Ken
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3214136/
https://ncbi.nlm.nih.gov/pubmed/22018137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-84
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