Bacterial Expression of a Human Monoclonal Antibody-Alkaline Phosphatase Conjugate Specific for Entamoeba histolytica

We previously produced human monoclonal antibody Fab fragments specific to Entamoeba histolytica in Escherichia coli. In order to use these Fab fragments for diagnostic purposes, an expression vector to produce a fusion protein of Fab and alkaline phosphatase (PhoA) in E. coli was designed and const...

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Bibliographic Details
Main Authors: Tachibana, Hiroshi, Takekoshi, Masataka, Cheng, Xun-Jia, Nakata, Yuta, Takeuchi, Tsutomu, Ihara, Seiji
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Microbial Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC321342/
https://ncbi.nlm.nih.gov/pubmed/14715571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.11.1.216-218.2004
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