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Light assisted antibody immobilization for bio-sensing

Ultrashort UV pulses at 258 nm with repetition rate of 10 kHz have been used to irradiate buffer solution of antibody. The tryptophan residues strongly absorb this radiation thus becoming capable to disrupt the disulfide bridges located next to them. Due to their high reactivity the opened bridges c...

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Detalhes bibliográficos
Main Authors: Della Ventura, Bartolomeo, Schiavo, Luigi, Altucci, Carlo, Esposito, Rosario, Velotta, Raffaele
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3207388/
https://ncbi.nlm.nih.gov/pubmed/22076280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.2.003223
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