Efficiency of Peptide Nucleic Acid-Directed PCR Clamping and Its Application in the Investigation of Natural Diets of the Japanese Eel Leptocephali

Polymerase chain reaction (PCR)-clamping using blocking primer and DNA-analogs, such as peptide nucleotide acid (PNA), may be used to selectively amplify target DNA for molecular diet analysis. We investigated PCR-clamping efficiency by studying PNA position and mismatch with complementary DNA by de...

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Main Authors: Terahara, Takeshi, Chow, Seinen, Kurogi, Hiroaki, Lee, Sun-Hee, Tsukamoto, Katsumi, Mochioka, Noritaka, Tanaka, Hideki, Takeyama, Haruko
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3206044/
https://ncbi.nlm.nih.gov/pubmed/22069444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0025715
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