Microproteomic Analysis of 10,000 Laser Captured Microdissected Breast Tumor Cells Using Short-Range Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Porous Layer Open Tubular (PLOT) LC-MS/MS

Precise proteomic profiling of limited levels of disease tissue represents an extremely challenging task. Here, we present an effective and reproducible microproteomic workflow for sample sizes of only 10,000 cells that integrates selective sample procurement via laser capture microdissection (LCM),...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Thakur, Dipak, Rejtar, Tomas, Wang, Dongdong, Bones, Jonathan, Cha, Sangwon, Clodfelder-Miller, Buffie, Richardson, Elizabeth, Binns, Shemeica, Dahiya, Sonika, Sgroi, Dennis, Karger, Barry L.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2011
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3205921/
https://ncbi.nlm.nih.gov/pubmed/21982995
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2011.09.022
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