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Detection, Evaluation and Minimization of Nonenzymatic Deamidation in Proteomic Sample Preparation

Identification of deamidated sites in proteins is commonly used for assignment of N-glycosylation sites. It is also important for assessing the role of deamidation in vivo. However, nonenzymatic deamidation occurs easily in peptides under conditions commonly used in treatment with trypsin and PNGase...

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Detalhes bibliográficos
Main Authors: Hao, Piliang, Ren, Yan, Alpert, Andrew J., Sze, Siu Kwan
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3205879/
https://ncbi.nlm.nih.gov/pubmed/21784994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O111.009381
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