Construction and partial characterization of two recombinant cDNA clones for procollagen from chicken cartilage.

Type II procollagen mRNA has been partially purified from embryonic chick sternal cartilage by guanidine hydrochloride extraction, sucrose gradient sedimentation and Sepharose 4B chromatography. Double stranded cDNA was synthesized using AMV reverse transcriptase and E. coli DNA polymerase I, tailed...

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Detalhes bibliográficos
Main Authors: Vuorio, E, Sandell, L, Kravis, D, Sheffield, V C, Vuorio, T, Dorfman, A, Upholt, W B
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320517/
https://ncbi.nlm.nih.gov/pubmed/6280134
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