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The gapped duplex DNA approach to oligonucleotide-directed mutation construction.
A simple and efficient method is described to introduce structurally pre-determined mutations into recombinant genomes of filamentous phage M13. The method rests on gapped duplex DNA (gdDNA) molecules of the phage M13 genome as the key intermediate. In this gdDNA, the (+) and the (shorter) (-) stran...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1984
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| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320472/ https://ncbi.nlm.nih.gov/pubmed/6096830 |
| Oznake: |
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