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Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull

We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demon...

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Detalhes bibliográficos
Main Authors: Drew, Patrick J., Shih, Andy Y., Driscoll, Jonathan D., Knutsen, Per Magne, Blinder, Pablo, Davalos, Dimitrios, Akassoglou, Katerina, Tsai, Philbert S., Kleinfeld, David
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3204312/
https://ncbi.nlm.nih.gov/pubmed/20966916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1530
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