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Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation

Endonucleases that generate double-strand breaks in DNA often possess two identical subunits related by rotational symmetry, arranged so that the active sites from each subunit act on opposite DNA strands. In contrast to many endonucleases, Type IIP restriction enzyme BcnI, which recognizes the pseu...

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Bibliografiske detaljer
Main Authors: Sasnauskas, Giedrius, Kostiuk, Georgij, Tamulaitis, Gintautas, Siksnys, Virginijus
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3203586/
https://ncbi.nlm.nih.gov/pubmed/21771860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr588
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