Cloning arg3, the gene for ornithine carbamoyltransferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid beta-lactamase in yeast.

The yeast arg3 gene, coding for ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase, EC 2.1.3.3), has been cloned on a hybrid pBR322-2-micrometers plasmid. The cloned gene gives a normal regulatory response in yeast. It is not expressed at 35 degrees C when a mutation...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Crabeel, M, Messenguy, F, Lacroute, F, Glansdorff, N
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1981
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320325/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7029528/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.8.5026
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