Polymer-stimulated ligation: enhanced ligation of oligo- and polynucleotides by T4 RNA ligase in polymer solutions.
The effects of macromolecular crowding were tested on several reactions catalyzed by T4 RNA ligase. The rate of cyclization of oligoriboadenylates was stimulated up to 10-fold by relatively high concentrations of several polymers (polyethylene glycol (PEG) 8000 or 20,000; bovine plasma albumin; Fico...
সংরক্ষণ করুন:
| প্রকাশিত: | Nucleic Acids Res |
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| প্রধান লেখক: | , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Oxford University Press
1984
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| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320305/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6504699/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.21.8235 |
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