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Altering Residues N125 and D149 Impacts Sugar Effector Binding and Allosteric Parameters in Escherichia coli Lactose Repressor
Lactose repressor protein (LacI), a negative transcriptional regulator in Escherichia coli, relies on an allosteric conformational change for its function. The LacI effector isopropyl-β,D-thiogalactoside (IPTG) promotes this allosteric response and engages the side chains of residues N125 and D149 b...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3202982/ https://ncbi.nlm.nih.gov/pubmed/21928765 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi200896t |
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