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Mechanism for Activation of Triosephosphate Isomerase by Phosphite Dianion: The Role of a Ligand-Driven Conformational Change

The L232A mutation at triosephosphate isomerase (TIM) from Trypanosoma brucei brucei results in a small 6-fold decrease in k(cat)/K(m) for the reversible enzyme-catalyzed isomerization of glyceraldehyde 3-phosphate to give dihydroxyacetone phosphate. By contrast, this mutation leads to a 17-fold inc...

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Hlavní autoři: Malabanan, M. Merced, Amyes, Tina L., Richard, John P.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3202976/
https://ncbi.nlm.nih.gov/pubmed/21939233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja208019p
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