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Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.

We have cloned the Escherichia coli tyrosine-inserting amber suppressor tRNA gene into the recombinant single-strand phage M12mp3. By using the M13mp3SuIII+ recombinant phage DNA as template and an oligonucleotide bearing a mismatch as primer, we have synthesized in vitro an M13mp3SuIII heteroduplex...

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Detalhes bibliográficos
Main Authors: Kudo, I, Leineweber, M, RajBhandary, U L
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC320241/
https://ncbi.nlm.nih.gov/pubmed/6170979
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