Loading...

Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.

We have cloned the Escherichia coli tyrosine-inserting amber suppressor tRNA gene into the recombinant single-strand phage M12mp3. By using the M13mp3SuIII+ recombinant phage DNA as template and an oligonucleotide bearing a mismatch as primer, we have synthesized in vitro an M13mp3SuIII heteroduplex...

Fuld beskrivelse

Na minha lista:
Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Kudo, I, Leineweber, M, RajBhandary, U L
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1981
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320241/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6170979/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.8.4753
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!