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Chromatin Immunoprecipitation (ChIP): Revisiting the Efficacy of Sample Preparation, Sonication, Quantification of Sheared DNA, and Analysis via PCR

The “quantitative” ChIP, a tool commonly used to study protein-DNA interactions in cells and tissue, is a difficult assay often plagued with technical error. We present, herein, the process required to merge multiple protocols into a quick, reliable and easy method and an approach to accurately quan...

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Detalhes bibliográficos
Main Authors: Schoppee Bortz, Pamela D., Wamhoff, Brian R.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201960/
https://ncbi.nlm.nih.gov/pubmed/22046253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0026015
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