A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90%...

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Autori principali: Corin, Karolina, Baaske, Philipp, Ravel, Deepali B., Song, Junyao, Brown, Emily, Wang, Xiaoqiang, Geissler, Sandra, Wienken, Christoph J., Jerabek-Willemsen, Moran, Duhr, Stefan, Braun, Dieter, Zhang, Shuguang
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2011
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201940/
https://ncbi.nlm.nih.gov/pubmed/22039398
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0023036
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