Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
We describe a method for the synthesis of microgram quantities of eucaryotic messenger RNAs. Injection into the cytoplasm of frog oocytes and addition to wheat germ extracts show that these synthetic RNAs function efficiently as messenger RNAs. We confirm that a 5' cap on the mRNA is essential for t...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1984
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC320142/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6207484/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.18.7057 |
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