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Cloning with tandem gene systems for high level gene expression.
A method has been devised for increasing the copy number of a gene (or genes) cloned into a plasmid while minimizing the size of the plasmid. If n copies of a transcriptional unit are cloned, including the promoter, coding region and terminator, the size of the plasmid will increase by n times the t...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1984
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320117/ https://ncbi.nlm.nih.gov/pubmed/6091039 |
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