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Gene A protein cleavage of recombinant plasmids containing the phi X174 replication origin.
Synthetic oligonucleotides, DNA ligase and DNA polymerase were used to construct double-stranded DNA fragments homologous to the first 25, 27 or 30 b.p. of the origin of replication of bacteriophage phi X174 (nucleotides 4299-4328 of the phi X174 DNA sequence). The double-stranded DNA fragments were...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1984
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320088/ https://ncbi.nlm.nih.gov/pubmed/6236428 |
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