Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.

A stopped-flow spectroscopic technique was used to study the kinetics of ion transport by reconstituted membrane preparations containing purified acetylcholine receptor. Influx of thallium (I) into membrane vesicles was monitored as a decrease, due to quenching by the thallous ion, in the fluorescen...

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Glavni autori: Wu, W C, Moore, H P, Raftery, M A
Format: Artigo
Jezik:Inglês
Izdano: 1981
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC319885/
https://ncbi.nlm.nih.gov/pubmed/6940146
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spelling pubmed-3198852004-03-13 Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor. Wu, W C Moore, H P Raftery, M A Proc Natl Acad Sci U S A Research Article A stopped-flow spectroscopic technique was used to study the kinetics of ion transport by reconstituted membrane preparations containing purified acetylcholine receptor. Influx of thallium (I) into membrane vesicles was monitored as a decrease, due to quenching by the thallous ion, in the fluorescence of an entrapped fluorophore. In a reproducible manner, the reconstituted receptor responded to cholinergic agonists by mediating rapid ion transport in the millisecond time range. The effect of agonists was blocked by receptor desensitization and by histrionicotoxin and was absent in membrane vesicles lacking receptor. Analysis of the fast kinetics of cation transport produced by saturating concentrations of agonists yielded an estimated rate of transport through a single reconstituted receptor channel. Comparison of this rate with those reported for in vivo preparations and for purified membranes shows that the reconstituted protein closely resembles the physiologically active receptor. 1981-02 /pmc/articles/PMC319885/ /pubmed/6940146 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
Wu, W C
Moore, H P
Raftery, M A
Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
description A stopped-flow spectroscopic technique was used to study the kinetics of ion transport by reconstituted membrane preparations containing purified acetylcholine receptor. Influx of thallium (I) into membrane vesicles was monitored as a decrease, due to quenching by the thallous ion, in the fluorescence of an entrapped fluorophore. In a reproducible manner, the reconstituted receptor responded to cholinergic agonists by mediating rapid ion transport in the millisecond time range. The effect of agonists was blocked by receptor desensitization and by histrionicotoxin and was absent in membrane vesicles lacking receptor. Analysis of the fast kinetics of cation transport produced by saturating concentrations of agonists yielded an estimated rate of transport through a single reconstituted receptor channel. Comparison of this rate with those reported for in vivo preparations and for purified membranes shows that the reconstituted protein closely resembles the physiologically active receptor.
author Wu, W C
Moore, H P
Raftery, M A
author_facet Wu, W C
Moore, H P
Raftery, M A
author_sort Wu, W C
title Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
title_short Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
title_full Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
title_fullStr Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
title_full_unstemmed Quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
title_sort quantitation of cation transport by reconstituted membrane vesicles containing purified acetylcholine receptor.
publishDate 1981
url https://ncbi.nlm.nih.gov/pmc/articles/PMC319885/
https://ncbi.nlm.nih.gov/pubmed/6940146
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